Articles on ByThom.com and Petapixel explaining how to get a current answer to your question, though I am sure that there is a lot more involved in your workflow than just Vuescan.
Apple menu>about this Mac>System Report>Software>Applications
From the list of applications that shows up (eventually) select them one by one. The data for that application will have the item "64 bit (Intel)" followed by either a 'yes' or a 'no'. If it is already 64 bit you are good to go. If the answer is 'no' you either have to hope/assume the developer will update the app when the time comes or you need to look for another application to use when that one will no longer work on the latest Mac-if your fave program gets stuck at 32 bit. It is not a bad idea to go through the entire list and make a note of every program which is not now 64 bit, just to know what's what.
Not sure what iteration your Vuescan is, but mine, which I no longer use for photos, only documents, is 9.1.2 and it is 32 Bit. I didn't check for any updates.
Silverfast is 64 Bit.
Adobe Camera RAW as others have mentioned is 32 Bit. Lots of things are 32 Bit. Lots of Nikon software apps are 32 Bit.
Interestingly enough, my Analog Efex Pro, Color Efex Pro, dFine2, HDR Efex Pro, Viveza 2, and Silver Efex Pro 2- my entire NIK Suite- shows up as 64 Bit on my computer.
Fotomagico, which I have heard on the web is 32 Bit, shows up as 64 Bit here.
If anyone has an answer to that puzzle, NIK is or is not 64 Bit, I'd be curious to hear. My computer thinks my NIK Suite is 64 Bit, that's all I know. Internet says it is 32 Bit.
People who have it loaded on a Mac, please check on your computer and maybe post the results. That might be interesting.
So, short answer to the OP's original question; it might effect your workflow, just depends on what other programs you use, but it is easy to check.